Publications

Links to publication databases:      

• Giuseppin, M.L.F.,  1984, Effects of dissolved oxygen concentration on lipase production by Rhizopus delemar. Appl. Microbiol. Biotechnol. 20:161-165.
• Giuseppin, M.L.F., van Eijk, H.M.J., Hellendoorn, M., van Almkerk, A.M.J., 1987, Cell wall strength of Hansenula polymorpha in continuous cultures in relation to the recovery of methanol oxidase (MOX). Appl. Microbiol. Biotechnol. 27: 31-6.
• Giuseppin, M.L.F., van Eijk, H.M.J., van Dijken, J.P., 1987. Production of a catalase-free oxidase and catalase-free oxidase containing yeast, and use thereof. WO87/07639.
• Giuseppin, M.L.F., 1988, PhD thesis, Optimization of methanol oxidase production by Hansenula polymorpha; an applied study on physiology and fermentation. Delft University of Technology, Delft.
• Giuseppin, M.L.F., van Eijk, H.M.J., Verduyn, C., Bante, I., van Dijken, J.P., 1988, Production of catalase-free alcohol oxidase by Hansenula polymorpha. Appl. Microbiol. Biotechnol. 28: 14-19.
• Giuseppin, M.L.F., van Eijk, H.M.J., Bes, B.C.M., 1988, Molecular regulation of methanol oxidase activity in continuous cultures of Hansenula polymorpha. Biotechnol. Bioeng. 32: 577-83.
• van Eijk, H.M.J., Giuseppin, M.L.F., Verduyn, C., Bante, I., van Dijken, J.P.,  ‘Production of Catalase-free Alcohol Oxidase’, in Proceedings 4th European Congress on Biotechnology 1987, O.M. Neijssel, R.R. van der Meer, K.Ch.A.M. Luyben (eds.),  Elsevier Science Publishers, 1987, p.p. 372 , ISBN 0-444-42829-1.
• Giuseppin, M.L.F., van Eijk, H.M.J., Bos, A., Verduyn, C., van Dijken, J.P., 1988, Utilization of methanol by a catalase-negative mutant of Hansenula polymorpha. Appl. Microbiol. Biotechnol. 28: 286-92.
• Verduyn, C., Giuseppin, M.L.F., Scheffers, W.A., van Dijken, J.P., 1988, Hydrogen peroxide metabolism in yeasts. Appl. Environ. Microbiol. 54: 2086-2090.  <+>
• Giuseppin, M.L.F., 1989, Mathematical modelling of growth and production of alcohol oxidase by Hansenula polymorpha grown on methanol-glucose mixtures. Biotechnol. Bioeng. 33: 524-35.
• Giuseppin, M.L.F., 1990, Preparation of catalase-free methanol oxidases from yeasts. EP0242007.
• Overbeeke, N., Termorshuizen, G.H.M., Giuseppin, M.L.F., Underwood, D.R., Verrips, C.T., 1990, Secretion of the  a-galactosidase from Cyamopsis tetragonoloba (guar) by Bacillus subtilis. Appl. Environ. Microbiol. 56: 1429-1434. <+>
• Giuseppin, M.L.F., Lopes, M.T.S., Planta, R.J., Verbakel, J.M.A., Verrips, C.T., 1991, Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector. Unilever N.V. WO9100920 A2.
• Veale, R. A., Giuseppin, M.L.F., van Eijk, H.M.J., Sudbery, P.E., Verrips, C.T., 1992. Development of a strain of Hansenula polymorpha for the efficient expression of guar a-galactosidase. Yeast. 8: 361-72.
• Giuseppin, M.L.F., Almkerk, J.W., Heistek, J.C., Verrips, C.T., 1993, Comparative study on the production of Guar a-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures. Appl. Env. Microbiol. 59: 52-59. <+> 

• Keulers, M., Ariaans, L., Giuseppin, M.L.F., 1994, The dynamic step response of a fed-batch bakers' yeast process. Prog. Biotechnol. 9: 879-882. (ECB6: Proc. 6th European Congress on Biotechnology, 1993, pt. 2),
• van den Berg, D.J.C., Robijn, G.W., Janssen, A.C., Giuseppin, M.L.F., Vreeker, R., Kamerling, J.P., Vliegenthart, J.F.G., Ledeboer, A.M., Verrips, C.T., 1995, Production of a novel extracellular polysaccharide by Lactobacillus-sake 0-1 and characterization of the polysaccharide. Appl.Env. Microbiol. 61:2840-2844. <get>
• Keulers, M., Ariaans, L., Soeterboek, R., Giuseppin, M., 1994, Facilitating control of fed-batch fermentation processes by monitoring the growth rates of Saccharomyces cerevisiae. Proceedings of the Third IEEE Conference on Control Applications. vol 3, 1935-1940. <+>
• BIOSPHERE, Giuseppin, M.L.F. (corporate project leader), "On-line real-time Knowledge Based Yeast Fermentation Control System", a Digital Twin advant la lettre, at Unilever Research Vlaardingen & Bolesian, 1994 -1995. <+>
• Adolph, S., Müller, S., Siedenberg, D., Jäger, K., Lehmann, H., Schügerl, K-H., Giuseppin, M.L.F., 1996, Cultivation of Aspergillus awamori on wheat bran: Ultrastructural description of growth and localization of xylanase by immunogold labeling. J. Biotechnol. 46:221-234.
• Freudenberg, S., Fasold, K-I., Müller, S., Siedenberg, D., Kretzmer, G., Schügerl, K-H., Giuseppin, M.L.F., 1996, Fluorescence microscopic investigation of Aspergillus awamori growing on synthetic and complex media and producing xylanase. J. Biotechnol. 46: 265-273.
• Domke, T., Nunn, C.C., Giuseppin, M.L.F., Martens, R.J., Swarthoff, T., Verrips, C.T., 1997, Enzymatic bleach composition. US5601750A.
• Siedenberg, D., Gerlach, S.R., Czwalinna, A., Schügerl, K-H., Giuseppin, M.L.F., Hunik, J., 1997, Production of xylanase by Aspergillus awamori on complex medium in stirred tank and airlift tower loop reactors. J. Biotechnol. 56: 205-216.
• Siedenberg, D., Gerlach, S.R., Weigel, B., Schügerl, K-H., Giuseppin, M.L.F., Hunik, J., 1997, Production of xylanase by Aspergillus awamori on synthetic medium in stirred tank and airlift tower loop reactors: the influence of stirrer speed and phosphate concentration. J. Biotechnol. 56: 103-114.
• van Riel, N.A.W., Giuseppin, M.L.F., ter Schure, E.G., Verrips, C.T., 1998, A structured, minimal parameter model of the central nitrogen metabolism in Saccharomyces cerevisiae which is able to predict the behaviour of mutants. J. Theor. Biol. 191: 397-414. <+>
• Cui, Y.Q., van der Lans, R.G.J.M., Giuseppin, M.L.F., Luyben, K.Ch.A.M.,1998, Influence of fermentation conditions and scale on the submerged fermentation of Aspergillus awamori. Enz. Microbiol. Technol. 23: 157-167.
• Cui, Y.Q., Ouwehand, J.N.W., van der Lans, R.G.J.M., Giuseppin, M.L.F., Luyben, K.Ch.A.M., 1998, Aspects of the use of complex media for submerged fermentation of Aspergillus awamori. Enz. Microbiol. Technol. 23: 168-177.
• van Riel, N.A.W., Giuseppin, M.L.F., Verrips, C.T., 1998, Use of Accelerostat to study slow dynamics of heterologous protein expression in S. cerevisiae. Conference Metabolic Engineering II, 25-30 October 1998, Elmau Germany. view 
• Gerlach, S.R., Siedenberg, D., Gerlach, D., Schügerl, K-H., Giuseppin, M.L.F., Hunik, J., 1998, Influence of reactor systems on the morphology of Aspergillus awamori. Application of neural network and cluster analysis for characterization of fungal morphology. Process Biochem. 33: 601-615. 
• Verrips, T., Duboc, P., Giuseppin, M., Sagt, C., 1998, Production of fungal cutinase with yeast. Proceedings of the EC Framework IV Symposium November 30 - December 2 1998, Eds. Osseweijer, P., van Dijken, J.P.,  Yeast as a Cell Factory. ISBN 90-9012242-7, pg 4. 
• van Riel, N.A.W., Saravane, A., Guillamon, J., Giuseppin, M.L.F., Verrips, C.T., 1998, Continuous culture studies and flux analysis of Δgdh1, Δgdh2 and Δglt1 mutants in S.cerevisiae CEN.PK strain. Proceedings of the EC Framework IV Symposium November 30 - December 2 1998, Eds. Osseweijer, P., van Dijken, J.P., Yeast as a Cell Factory. ISBN 90-9012242-7, pg 75-76.
• Giuseppin, M.L.F., Verrips, C.T., van Riel, N.A.W., 1999, The cell-factory needs a model of a factory. TIBTECH 17: 383-384.<+>
• Giuseppin, M.L.F., van Riel, N.A.W., 2000, Metabolic modeling of Saccharomyces cerevisiae using the optimal control of homeostasis: a cybernetic model definition. Metabolic. Eng. 2: 14-33.<+>
• van Riel, N.A.W., Giuseppin, M.L.F., Verrips, C.T., 2000, Dynamic optimal control of homeostasis: an integrative system approach for modeling of the central nitrogen metabolism in Saccharomyces cerevisiae. Metabolic Eng. 2: 49-68.<+>   
• Dijken, J.P. van, Bauer, J., Brambilla, L., Duboc P., Francois, J.M., Gancedo, C., Giuseppin, M.L.F., Heijnen, J.J., Hoare, M., Lange, H.C., Madden, E.A., Niederberger, P., Nielsen, J., Parrou, J.L., Petit, T., Porro, D., Reuss, M., van Riel, N.A.W., Rizzi,  M., Steensma, H.Y., Verrips, C.T., Vindeløv, J., Pronk, J.T., 2000, An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb. Technol. 26: 706-714.<+>  
• Giuseppin, M. L.F., Ouwehand, J., Bellissen, L., Ter Schure, E., 2000, Fermented, Pasteurised Preferment. EP1094715. 
• van der Sluis, C., Wolken, W.A.M., Giuseppin, M.L.F., Tramper, J., Wijffels, R.H., 2000, Effect of threonine, cystathionine, and the branched-chain amino acids on the metabolism of Zygosaccharomyces rouxii. Enz. and Microb. Technol., 26: 292-300.
• van der Sluis, C., Westerink, B.H., Dijkstal, M.M., Castelein, S.J., van Boxtel, J.B., Giuseppin, M.L.F., Tramper, J., Wijffels, R.H., 2001, Estimation of steady-state culture characteristics during acceleration–stats with yeasts. Biotechnol. Bioeng. 75: 269-275. 
• Guillamón, J.M., van Riel, N.A.W., Giuseppin, M.L.F., Verrips, C.T., 2001, The glutamate synthase (GOGAT) of Saccharomyces cerevisiae plays an important role in the central nitrogen metabolism. FEMS Yeast Research. 1: 169-175. <+>

• Mooibroek, H., Oosterhuis, N., Giuseppin, M., Toonen, M., Franssen, H., Scott, E., Sanders, J., Steinbüchel, A., 2007, Assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid  production. Appl. Microbiol. Biotechnol. 77: 257-267. <+>  
• Giuseppin, M.L.F., van der Sluis, C., Laus, M.C., 2008, Potato protein isolates. WO2008069650.
• Bisschops, M.A.T., Giuseppin, M.L.F., 2008, Method and device for continuous chromatographic separations. EP 1994972.
• Bisschops, M.A.T., Giuseppin, M., Horneman, D., 2008, Continuous Recovery of Proteins using Large-scale Multicolumn EBA Chromatography Techology.Conference Recovery of Biological products XIII, Quebec, Canada, 22-27 June.
• Giuseppin, M.L.F., Bakker, W., 2008, Protein gel formation. WO 2008069649.
• Giuseppin, M.L.F., Laus, M.C., 2008, Glycoalkaloid removal. WO 2008056977.
• Giuseppin, M.L.F., Spelbrink, R.E.J., 2008, Glycoalkaloid removal, WO 2008069651.
• Giuseppin, M.L.F., Spelbrink, R.E.J., 2009, Method for preparing food product. WO2009061186.
• Klijnstra, G., Giuseppin, M.L.F., 2010, Enhanced beer foaming. WO 2010062174.
• Giuseppin, M.L.F., Nieuwenhuijzen, N.H., Tromp, R.H., 2010, Condiment. WO2010098663.
• Giuseppin, M.L.F., Nieuwenhuijzen, N.H., Lambers, T.T., Spelbrink, R.E.J., 2011, Non-astringent protein products. WO2011059330.
• Giuseppin, M.L.F., Smits, P.J., Hofland, G.W., 2011, Sub-critical gas assisted drying of biopolymer material. WO2011102723.
• Creusot, N., Wierenga, P.A., Laus, M.C., Giuseppin, M.L., Gruppen, H., 2011, Rheological properties of patatin gels compared with ß-lactoglobulin, ovalbumin, and glycinin. J. Sci. Food Agric. 91(2): 253-61. <+>
• Alting, A.C., Pouvreau, L., Giuseppin, M.L.F., Nieuwenhuijzen, N.H., 2011, Potato proteins. in: Handbook of Food Proteins, Eds. G.O. Phillips and P.A. Williams, Woodhead Publishing, p316-334, ISBN 1-84569-758-8. <+>
• Spelbrink, R.E.J., Gerrits, P.J., Mooij, C., Giuseppin, M.L.F.,  2011, Quantitative Determination of Trypsin Inhibitory Activity in Complex Matrices. The Open Food Science Journal.5:37-41. <+>
• Reijers, R., Giuseppin, M., Sotiriadou, A., Houben, R., 2012, Innovative technology for drying potato proteins agitated thin film dryer (ATFD), Poster 9th European Congress of Chemical Engineering (ECCE9) and 2nd European Congress of Applied Biotechnology (ECAB2), The Hague 21-24 April 2013. <+>
• Lynch, B., Simon, R.R., van Otterdijk, F.M., Emmen, H.H., Giuseppin, M.L.F., Kemme-Kroonsberg, C., 2012, Subchronic toxicity evaluation of potato protein isolates. Food and Chemical Toxicology, 50(2): 373-384. <+>
• Solanic BV, 2012, GRAS Notice 447, Potato protein isolates, filed Nov 5 2012. <+>
• He, T., Spelbrink, R.E.J., Witteman, B.J., Giuseppin, M.L.F., 2013, Digestion kinetics of potato protein isolates in vitro and in vivo. J. Food Sci. Nutrition. 64(7): 787-793. <+>
• Delahaije, R.J., Wierenga, P.A., van Nieuwenhuijzen, N.H., Giuseppin, M.L., Gruppen, H., 2013, Protein concentration and protein-exposed hydrophobicity as dominant parameters determining the flocculation of protein-stabilized oil-in-water emulsions. Langmuir. 29(37): 11567-74.  <+>
• He, T., Giuseppin, M.L.F., 2014, Slow and fast dietary proteins differentially modulate postprandial metabolism. Int. J. Food Sci. Nutr. 65(3): 386-390.
• Delahaije, Roy J.B.M.,  Gruppen, Harry, van Nieuwenhuijzen, Neleke H., Giuseppin, Marco L.F.,  Wierenga, Peter A., 2013, Effect of Glycation on the Flocculation Behavior of Protein-Stabilized Oil-in-Water Emulsions, Langmuir, 29(49):15201–15208. <+>
• Spelbrink, R.E.J., Giuseppin, M.L.F., Egmond, M.R., 2014, Methods using patatin, WO2014007621 A1.
• Giuseppin, M.L.F., Laus, M.C., Schipper, J., 2014, Potato protein Isolates. WO2014011042.
• Spelbrink, R.E., Giuseppin, M.L., 2014, Large-scale single step partial purification of potato pectin methylesterase that enables the use in major food applications. Appl. Biochem. Biotechnol. 174(5): 1998-2006. <+>
• Delahaije, R.J.B.M., Gruppen, H., Giuseppin, M.L.F., Wierenga, P.A., 2014, Quantitative description of the parameters affecting the adsorption behaviour of globular proteins. Colloids and Surfaces B: Biointerfaces 123: 199- 206. <+>
• Delahaije, R.J.B.M., Wierenga, P.A., Giuseppin, M.L.F., Gruppen, H., 2014, Improved emulsion stability by succinylation of patatin is caused by partial unfolding rather than charge effects. J. Colloid and Interface Science, 430: 69-77. <+>
• Delahaije, R.J.B.M., Gruppen H., Giuseppin Marco L.F., Wierenga Peter A., 2015, Towards predicting the stability of protein-stabilized emulsions. Adv. Colloid Interface Sci. 219:1-9.<+>
• Delahaije, R.J.B.M., Wierenga, P.A., Giuseppin, M.L.F., Gruppen, H., 2015, Comparison of Heat-Induced Aggregation of Globular Proteins. J. Agric. Food Chem.  63(21): 5257-5265. <+>
• Spelbrink, R.E., Lensing, H., Egmond, M.R., Giuseppin, M.L., 2015, Potato Patatin Generates Short-Chain Fatty Acids from Milk Fat that Contribute to Flavour Development in Cheese Ripening. Appl Biochem Biotechnol. 176: 231-243. <+>
• Giuseppin, M.L.F., Spelbrink, R.E.J. Mooij, C.M.A., 2015, Production of yoghurt. WO2015170985 A1.
• Giuseppin, M.L.F., Spelbrink, R.E.J., Laus, M.C., Schipper, J., 2016, Flocculation, WO2016036243 A1. 
• Giuseppin, M.L.F., Iannacone, S., Koopmans, W., Spelbrink, R.E.J., Berghout, J.A.,M., 2017, Freeze concentration of root- or tuber juice and derived products. WO2017146568 A1.
• Giuseppin, M.L.F., Mooij, C.M.A., Spelbrink, R.E.J., 2017, Fermentation; Enhancers of microbial growth, WO2017078530 A1. 

• Wetsus, European centre of excellence for sustainable water technology, Leeuwarden, Senior advisor, 2019-2021

• no publications.